We've updated our privacy policy. Sample collection for bacterial isolation, characterization and antibiotic se Lecture 12-laboratory and field investigation, Collection and Handling of Specimens for Laboratory Diagnosis, Collection of biological specimens for microbiology tests, Collection, Preservation and Dispatch of Clinical Samples, U. P. 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Uses of laboratory test To provide information relating to a clinical diagnosis To investigate the etiology of a clinical condition To study complications of a disease or its treatment To provide prognostic information To monitor natural history or response to Rx. Most cellular and macromolecular analytes are 5 to 15% higher when specimens are collected erect as compared to supine. For certain tests, 24-hour urine samples are collected at home and must be refrigerated during the collection process. multiple methods (routine diagnostic, serology, bacteremia is sometimes transient growth may, unless multiple samples are collected, it is, Sufficient quantity of specimen is required, Swabs, due to the small volume collected and the, The quicker the specimen is processed the better, Pathogens may die in transit thus leading to, If transport is delayed, a transport medium, a reducing agent such as thioglycolate can be, CO2 can be included to support viability of, Charcoal, gelatin, or corn starch can be included, Buffered glycerol is sometimes used to transport, Boric acid will preserve urine specimens for, Refrigerated urine for culture can be kept up to, Some microbes do not survive well even in, Neisseria gonorrhoeae lose viability genital, If swabs must be used the following guidelines, Cotton may contain toxins to fastidious pathogens, Calcium alginate inhibits Chlamydia and other, Dacron (polyester) is the best all-around, The upper respiratory tract (URT) includes the, The oral cavity, oropharynx nasopharynx, are, The eustachian tube, middle ear, and the nasal, The principle of collecting a throat specimen is, A tongue blade should be used to press down on, The throat should be visualized using good, Areas in the posterior pharynx that appear, The tonsilar fossae and the extreme posterior. Collect skin scrapings in a dry sterile container. Arthropod (Tick) for ID. - The research report includes specific segments by region (country), by company, by Type and by Application. endobj 6. A request form should include the following information: Sooner the specimens reach the laboratory the better. The specimen should be collected in the morning to reach the laboratory before noon, so that it can be processed the same day. The majority of our products are designed for automation further simplifying your workflow and eliminating the need for multiple suppliers. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. PowerShow.com is brought to you byCrystalGraphics, the award-winning developer and market-leading publisher of rich-media enhancement products for presentations. An exception to this rule, throat swabs for S. Since the indigenous microbes are almost always, Also, what is normal in the oropharynx may be, Each URT site can be infected with a variety of, The site most commonly infected is the oropharynx, Pharyngitis is most frequently caused by viruses, Viral phayngitis cultures are not rountinely, The exception to this rule is RSV which can be, Routine cultures of the oropharynx in the U.S.A, S. pyogenes pharyngitis is frequently associated, S. pyogenes can readily be detected by culturing, Some studies have shown that the use of a, SBA containing antibiotics (e.g trimethoprim, A moistened swab on a thin flexible wire is used, It is inserted through one of the nares and slid, When the swab reaches the nasopharyngeal chamber, The procedure is repeated using the same swab in, If C. diptheriae (diphtheria) or Bordetella, Corynebacterium diptheriae (diphtheria) and, However, some individuals are not immune. During vegetation sampling, collect repre- before you begin. Patient's Medical record Number . Collect the sample in the appropriate container. Specimen Transportation a. Heparin (Anti-Coagulant) Used to collect whole blood or plasma Binds to anti-thrombin III to inhibit Xa, IXa, and thrombin Nominal concentration of 12 30 U/mL Heparin binds calcium so ionized calcium must be collected using Calcium Titrated or Electrolyte Balanced heparin, Citrate (Anti-Coagulant) Citrate is used for collection of coagulation tests Acts by binding calcium Nominal concentration of 3.2% (0.109 mol/L) Recent move to 3.2% from 3.8% to get more consistent results for Prothrombin Time, particularly for more sensitive reagents Tubes must be properly filled to within +/- 10% of assigned collection volume, Clot Activator Shortens Time to Clot In vitro activation of clotting system to enhance clot formation A silica clot activator is attached to the tube wall with a silicone surfactant Requires mixing for optimal affect Time to clot is 15-30 minutes instead of 1 hour, Serum Separator Gel Separates Serum from Cells Polymer gel with specific gravity between that of serum and cells Migrates and forms a barrier during centrifugation Separation of cells from serum stops metabolic and hemolytic effects Certain analytes and therapeutic drugs may bind to gel over time, Labelling the Sample A properly labelled sample is essential so that the results of the test match the patient. Specimen labelling and processing: Name and address of the hospital or health centre. 1 COLLECTION OF SPECIMENS Collection of botanical material involves two activitiesgathering the specimens and recording the information. What containers are required to collect the specimens? Looks like youve clipped this slide to already. Viruses can also be damaged be light, drying, change in pH, and bacterial enzymes. Tube slips back from vacutainer needle. SAA provides many . All specimen containers must be transported in a double-sided, self-sealing polythene bag with one compartment containing the laboratory request form and the other the specimen. Title: Microsoft PowerPoint - sample collection [Compatibility Mode] Author: Nishi Created Date: 5/25/2017 6:51:58 PM . TCZ9S 'i2&M;2.AeD6$?` x - BD (Becton Dickinson) is a worldwide leader in blood sample collection tubes. The health technician logs onto the specimen collection application and logs the SP into the component. Sensitivity which antibiotics are effective, Start stream, then stop, collect specimen, Note start time on container requisition, Single positive test result does not confirm. - TBRC global virology specimen collection market report includes blood collection kits, specimen collection tubes, viral transport media, swabs. Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. Gloves - can be made of latex, rubber, vinyl, etc. Two to. If a feces sample can not be obtained, a rectal swab should be collected and transported in buffered saline. Previously parataxonomy of these titanosaur clutches . Concepts of Specimen Collection Importance of Specimen Collection Nurses' Roles in Specimen Collection 1. Specimen Collection and Transport. Perform the venipuncture. Methodologies and Regulations in Specimen Collection and Management - . . By accepting, you agree to the updated privacy policy. Use the container/tube indicated in the test requirements for appropriate specimen preservation. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. Then you can share it with your target audience as well as PowerShow.coms millions of monthly visitors. Else beotalk 1200 handleiding 13 infinity road. We've encountered a problem, please try again. collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) (ii) For special cases (examination of endocrines and viral infection-like PPR) tissues are to be collected in Bouin's fluid. Viruses are unable to survive temperature over 50C, freezing or fluctuating in temperature. Specimen collection 1: general principles and procedure for obtaining a midstream urine specimen | Nursing Times EMAP Publishing Limited Company number 7880758 (England & Wales) Registered address: 10th Floor, Southern House, Wellesley Grove, Croydon, CR0 1XG We use cookies to personalize and improve your experience on our site. WHAT IS THE MOST IMPORTANT THING YOU NEED FOR SPECIMEN COLLECTION??? lesson objectives. A four-fold rise in titer between paired sera establishes a positive result. Always immediately label each specimen before leaving the patient (at bed side). 5-3: Collection and preservation The collection of appropriate and optimum samples is the responsibility of the laboratory, even though the actual collection process is often carried out by persons . foundation block (2018). This guidance includes information on each step shown below. Proper specimen labeling. Some sex hormones need special timing as regard cycle : (1)FSH,LH (3rd day of cycal {follicular phase}) (2)Progesterone (21 day of cycle{ luteal phase}) Other Factors: Age, gender, and pregnancy have an influence on laboratory testing. laboratory diagnosis of, Diagnostic Sampling - . Adhesive bandages / tape - protects the venipuncture site after collection, Collection Tube Additives Heparin EDTA Citrate Clot Activator Serum Separator, Blood should NEVER be poured from one tube to another since the tubes can have different additives or coatings. specimen collection, preservation and transportation ppt Maintaining any specimen for many years carries a cost. endobj Insertion of an in and out catheter just to collect the specimen. Avoid drawing blood from a hematoma, IVs and catheters Draw slowly when collecting with syringes Avoid drawing the plunger back too forcefully, and avoid frothing of the sample. MGM medical college ,mumbai PowerPoint PPT presentation, Bacterial and Viral Specimen Collection Market, - Bacterial and Viral Specimen Collection Market, | PowerPoint PPT presentation | free to view, The bacterial and viral specimen collection market was estimated at USD 13.30 billion in 2019, - The bacterial and viral specimen collection market was estimated at USD 13.30 billion in 2019, Virology Specimen Collection MarketReport 2022- Market Size, Trends, And Global Forecast 2022-2031. What specimens are to be collected? Serena Lobo 5. prior to antibiotics or dosing schedules). You can read the details below. If measles or rubella is suspected, the virus is more likely to be isolated from a paranasal swab. hmO9_6~?UUw:-'!^l*lP'c=cg~cJ5IDNhE 4zBCIda0 endobj Perform the venipuncture. Think like a microbe. Viral Specimens: Collection, Transportation and Preservation for Laboratory Diagnosis of Viruses Viral Specimens collection Viral Specimens or samples collection plays a vital role in laboratory diagnosis. <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Aspirate . Serum Separator tubes should also be inverted. Urine collection. 282-288, 297-298, 360-361 and 380-381 - . Effects of Exercise: Muscular activity has both transient and longer lasting effects. This requisition form must be reviewed to understand What tests are requested? The absence of resin, which was apparently not used during the preparation of the mummies, and the almost complete . Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. Diagnosis. NOTE: Both of the above MUST match the same on the requisition form. The site is a companion to the museum's new human origins exhibit. 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Health technician logs onto the specimen should be collected and transported in buffered saline 5 to %... In pH, and bacterial enzymes preservation and transportation ppt Maintaining any specimen for many years carries a..
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